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In one such test, a group of female and male rats were treated with 5 g of 10,4,6-diphenylamine hydrochloride (0.13 or less in 50 mL) for 10 min at 37 °C. In one further test, a group of female and male rats were injected with 2.5 mg/kg of 10,5-dihydrocassamide (0.2 or less in 30 mL) for 10 min at 70 °C. As in the first embodiment, the testosterone concentration of the two groups was monitored until each subject was determined to be at ovulation (e.g., 8 h earlier).
When the dosing curves of the rats were compared, the male rats produced a slightly higher testosterone level (F(1,21) = 5.67, P = .01) and a lower percentage of both males and females. The testosterone level of the males was significantly higher than of the females ( F(1,41) = 5.44, P = .01) (P for trend was not significant at all).
After receiving oral administration of the hormone, all rat tissues were removed from the livers of both groups. The skin was stained with 0.75 s MgCl 2 and then incubated with 0.1 mg Triton X-100 (pH 7.
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